CERTIFICATE OF ANALYSIS
50bp DNA Ladder
Concentration: for 100 applications
Supplied with: 1ml 6xLoading Dye Solution
Store: store at -20℃.
GeneRuler 50bp DNA Ladder is prepared from a specially designed plasmid pEJ3 DNA, containing pUC, λ phage and yeast genome sequences.
pEJ3 DNA was completely digested with Eco 147I and Pvul. The DNA Ladder is in storage buffer. The DNA Ladder contains the following 13 discrete fragments (in base pairs): 1031, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50. dissolved
10mM Tris-HCI (pH7.6), 1mM EDTA.
6x Loading Dye Solution
10mM Tris-HCI (pH 7.6), 0.03% bromophenol blue,
0.03% xylene cyanol FF, 60% glycerol and 60mM EDTA
Quality Control Assay Data
Analysis of 0.5ug (1ul) of the DNA Ladder on agrose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete bands pattern.
Quality authorized by: Jurgita Zilinskiene
RECOMMENDATIONS FOR USE
- Prepare the DNA Ladder before loading:
1ul (0.5ug) of the DNA Ladder,
1ul of 6 x Loading Dye Solution,
4ul of deionized water;
-vortex gently just prior to use;
-do not heat before loading,
-apply the prepared amount (6ul) of the DNA Ladder on a 5mm lane of agarose gel;
-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.