100bp plus

CERTIFICATE OF ANALYSIS

GeneRuler

100bp DNA Ladder Plus

Ready-to-use 

#65-0321 

Application: 100 applications 

Lot:                  2801

Supplied with: 6x loading Dye 

                         Solution                                                              

Store:                  at room temperature at                                    

                            .+4 ℃   

Description

GeneRuler 100bp DNA Ladder Plus is prepared from three different plasmids, containing pUC,pBR322. lamda phage and yeast genome sequences. Which were individually digested with appropriate restriction endonucleases, phenol extracted. Ethanol precipitated, dissolved in 10mM Tris-HCI (pH7.6) and 1mM EDTA. 

The DNA Ladder contains the following 14 discrete fragments (in base pairs):3000,2000,1500,1200, 1031,900,800,700,600,500,400, 300,200,100. 

Supplied in Storage Buffer

10mM Tris-HCI (pH7.6), 1mM EDTA. 

0.015%bromophenol blue, 0.015% xylene cyanol FF and 10% glycerol. 

6x Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA. 

Quality Control Assay Data

Agarose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns. 

Quality authorized by: Linda Muller  

RECOMMENDATIONS FOR USE

Vortex gently just prior to use. 

Do not heat before loading 

Apply 6ul-10ul of DNA Ladder per line of mini agarose gel . 

Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining. 

1ug of the GeneRuler  100bp DNA Ladder Plus contains 102ng (10%) of the 5000bp fragment.