CERTIFICATE OF ANALYSIS
100bp DNA Ladder Plus
Application: 100 applications
Supplied with: 6x loading Dye
Store: at room temperature at
GeneRuler 100bp DNA Ladder Plus is prepared from three different plasmids, containing pUC,pBR322. lamda phage and yeast genome sequences. Which were individually digested with appropriate restriction endonucleases, phenol extracted. Ethanol precipitated, dissolved in 10mM Tris-HCI (pH7.6) and 1mM EDTA.
The DNA Ladder contains the following 14 discrete fragments (in base pairs):3000,2000,1500,1200, 1031,900,800,700,600,500,400, 300,200,100.
Supplied in Storage Buffer
10mM Tris-HCI (pH7.6), 1mM EDTA.
0.015%bromophenol blue, 0.015% xylene cyanol FF and 10% glycerol.
6x Loading Dye Solution
0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA.
Quality Control Assay Data
Agarose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns.
Quality authorized by: Linda Muller
RECOMMENDATIONS FOR USE
Vortex gently just prior to use.
Do not heat before loading
Apply 6ul-10ul of DNA Ladder per line of mini agarose gel .
Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining.
1ug of the GeneRuler 100bp DNA Ladder Plus contains 102ng (10%) of the 5000bp fragment.
|Product Name||100bp plus|