1kb

CERTIFICATE OF ANALYSIS

GeneRuler

1kb DNA Ladder 

Ready-to-use 

#65-0311 

Application: 100 Applications 

Lot:                  VA08

Store:                  at room temperature at +4 ℃ 

                            For periods up to 6 months. 

                            For longer periods store at -20 ℃. 

Description

GeneRuler 1kb DNA Ladder is supplied in gel loading buffer at total DNA concentration of 0.1ug/ul and can be directly applied on to agarose gel. This DNA Ladder is prepared from six different plasmids, containing pUC, lamda phage and yeast genome sequences. 

The DNA Ladder contains the following 14 discrete fragments (in base pairs): 10000,8000,6000,5000,4000, 3500,3000,2500,2000,1500,1000, 750,500,250. 

Supplied in Gel Loading Buffer

10mM Tris-HCI (pH7.6), 10mM EDTA. 

0.015%bromophenol blue, 0.015% xylene cyanol FF and 10% glycerol. 

6x Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA. 

Quality Control Assay Data

Agarose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns. 

Quality authorized by: Linda Muller  

RECOMMENDATIONS FOR USE

Vortex gently just prior to use. 

Do not heat before loading 

Apply 6ul of DNA Ladder per line of mini agarose gel. 

Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining. 

1ug of the GeneRuler  1kb DNA Ladder, ready-to-use, can be contains ≡310ng (≡31%) of the 3000bp fragment.