PUC Mix Marker, 8

CERTIFICATE OF ANALYSIS

pUC Mix Marker, 8  

Ready-to-use  

#65-0301 50ug 

( 100 applications)  

Concentration: 0.5ug/ul  

Lot:                   1101  

Supplied with: 1ml 6x Loading Dye           Solution  

Store:           store at -20 ℃.  

Description

pUC19DNA and pUC57 DNA were individually completely digested with Mspl and Dral+Hindlll respectively, purified and dissolved in a storage buffer. 

All digests were then mixed. 

The DNA Marker contains the following 17 

Discrete fragments (in base pairs): 1118, 881, 692, 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26, 19.  

Storage Buffer

10mM Tris-HCI (pH7.6), 1mM EDTA.  

6x Loading Dye Solution

10mM Tris-HCI (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60mM EDTA.  

Quality Control Assay Data

Agarose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns.  

RECOMMENDATIONS FOR USE  

Loading on agarose gel

-Loading 6ul of the read-load marker on a 5mm lane of agarose gel. 

-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.  

Note

-One vial (50ug/600ul)is sufficient for ~100 applications.