CERTIFICATE OF ANALYSIS
pUC Mix Marker, 8
( 100 applications)
Supplied with: 1ml 6x Loading Dye Solution
Store: store at -20 ℃.
pUC19DNA and pUC57 DNA were individually completely digested with Mspl and Dral+Hindlll respectively, purified and dissolved in a storage buffer.
All digests were then mixed.
The DNA Marker contains the following 17
Discrete fragments (in base pairs): 1118, 881, 692, 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26, 19.
10mM Tris-HCI (pH7.6), 1mM EDTA.
6x Loading Dye Solution
10mM Tris-HCI (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60mM EDTA.
Quality Control Assay Data
Agarose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns.
RECOMMENDATIONS FOR USE
Loading on agarose gel
-Loading 6ul of the read-load marker on a 5mm lane of agarose gel.
-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.
-One vial (50ug/600ul)is sufficient for ~100 applications.
|Product Name||PUC Mix Marker, 8|