PUC DNA/Mspl Marker, 23

CERTIFICATE OF ANALYSIS 

pUC19 DNA/MspI (HpaII) 

Marker, 23 

Ready-to-use 

#65-0221 

50ug (100 applications) 

Concentration: 0.5ug/ul 

Lot:                   2101 

Supplied with: 1ml 6x Loading Dye Solution 

Store:           store at -20 ℃ 

Description 

Puc19DNA was completely digested with Mspl, purified and dissolved in a storage buffer. 

The DNA Marker contains the following 13 

Discrete fragments (in base pairs): 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26. 

Storage Buffer 

10mM Tris-HCI (pH7.6), 1mM EDTA. 

6x Loading Dye Solution 

0.2% bromophenol blue, 0.2% xylene cyanol FF, 60% glycerol and 60mM EDTA. 

Quality Control Assay Data 

Agarose gel electrophoretic analysis of homogeneity of Mspl fragmentation patterns. 

RECOMMENDATIONS FOR USE 

Loading on agarose gel 

-Loading 6ul of the read-load marker on a 5mm lane of agarose gel. 

-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining. 

Note 

-One vial (50ug/600ul)is sufficient for ~100 applications.