CERTIFICATE OF ANALYSIS
pUC19 DNA/MspI (HpaII)
Marker, 23
Ready-to-use
#65-0221
50ug (100 applications)
Concentration: 0.5ug/ul
Lot: 2101
Supplied with: 1ml 6x Loading Dye Solution
Store: store at -20 ℃
Description
Puc19DNA was completely digested with Mspl, purified and dissolved in a storage buffer.
The DNA Marker contains the following 13
Discrete fragments (in base pairs): 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26.
Storage Buffer
10mM Tris-HCI (pH7.6), 1mM EDTA.
6x Loading Dye Solution
0.2% bromophenol blue, 0.2% xylene cyanol FF, 60% glycerol and 60mM EDTA.
Quality Control Assay Data
Agarose gel electrophoretic analysis of homogeneity of Mspl fragmentation patterns.
RECOMMENDATIONS FOR USE
Loading on agarose gel
-Loading 6ul of the read-load marker on a 5mm lane of agarose gel.
-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.
Note
-One vial (50ug/600ul)is sufficient for ~100 applications.
Product Name | PUC DNA/Mspl Marker, 23 |
---|---|
SKU | 65-0221 |