CERTIFICATE OF ANALYSIS
Prestained Protein Ladder
#65-0671 2x250ul Ready-Load
(for 100 mini-gel applications 5ul per well or 50 large gel applications 10ul per well)
Lot: 5929 Quality guaranteed: 12.2009
Store at -20℃
PageRuler Prestained Protein Ladder is a mixture of 10 recombinant, highly purified colored proteins with the apparent molecular weights from 10kDa to 170kDa. It is designed for monitoring of protein separation during SDS-PAGE, verifying Western transfer efficiency and approximating the molecular weight of blotted proteins (1-3). Proteins are covalently coupled with a blue chromophore except for a reference one- 72kDa, coupled with an orange dye. The PageRuler Prestained Protein Ladder is supplied in gel loading buffer and is ready-to-use.
Approximately 0.1-0.2mg/ml of each protein in the storage buffer (62.5mM Tris-H3PO4 (pH 7.5 at 25), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol).
Instruction for Use
5ul per well for mini-gels, 3ul per well for blots,
10ul per well for large gels, 6ul per well for blots.
|Product Name||10-170 kDa Pre-Stained Protein Marker / 2 x 250ul|