CERTIFICATE OF ANALYSIS
Protein Ladder, 10-200kDa
Lot: 0201 Quality guaranteed: 05. 2006
Store at -20℃
Protein Ladder, 10-200kDa, is a mixture of 14 recombinant, highly purified proteins designed for precise sizing of proteins by SDS-PAGE. The proteins resolve into clearly identifiable sharp bands from 10kDa to 200kDa when analyzed by SDS-PAGE and stained with Coomassie Blue (1). The Protein Ladder is supplied in gel loading buffer and is ready-to-use. Each protein contains an integral Strep-tag® II sequence and may be detected on Western blots using Strep-Tactin® -AP conjugate or antibody against Strep-tag® II (2,3).
Approximately 0.02-0.05mg/ml of each protein in the following storage buffer (62.5mM Tris-H3PO4 (pH 7.5 at 25), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol).
Precise molecular weight determination of polypeptides in denaturing PAA gels and Western blots (1, 4).
Instruction for Use
5ul for mini-gels, 3ul for blots,
10ul for large gels, 6ul for blots.
Western transfer procedure as desired.
The 50k Da protein band is of greater intensity and serves as a reference.
Loading volumes are indicated for in gels with a thickness of 0.75mm. If thicker gels are used, the loading volume should be increased.
When Western blots are indicated for in gels with a thickness of 0.75mm. If thicker gels are used, the loading volume should be increased.
When Western blots are performed, Prestained Protein Ladder (#65-0671) might be used for monitoring of transfer efficiency.
The 200kDa band has lower intensity than the other bands when detected on Western blots using Strep-Tactin® -AP conjugate.
The Protein Ladder is optimized for use with Coomassie Brilliant Blue R-250 staining. For silver staining the amount of Protein Ladder applied might be decreased up to 10 times.
|Product Name||10-200 kDa Unstained Protein Marker|