RNA Marker High Range

CERTIFICATE OF ANALYSIS

RNA Ladder, High Range

Ready-to-use

#65-0412    600ul

                        (for 100 applications)

Lot: 9625   Quality guaranteed: 03.2010

Concentration: 0.5ug/ul

Supplied with: 1ml 2X Loading Dye Solution                                             

Store:       at -70℃ or -20℃. (up to 6 months) 

Description

RNA Ladder, High range, is a set of seven RNA transcripts with lengths of 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200 bases. RNA transcripts are produced from specific templates, containing a fragment of the pTZ19R polylinker and λ phage sequences. The RNA Ladder Range is suitable for analysis of RNA in native or denaturing agarose gels and can be easily visualized by ethidium bromide staining.

Storage Buffer

20mM potassium acetate (pH 4.5)

2x Loading Dye Solution

95% formamide, 0.025% SDS,

0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, 0.5mM EDTA.

Quality Control Assay Data

Analysis of 1ug (2 ul)of RNA Ladder, High Range, on a native agarose gel with ethidium bromide staining generates a clear eight-band pattern.

Quality authorized by: Linda Sun

RECOMMENDATIONS FOR USE

Thaw the marker at room temperature or heat at 37-40℃ for a few minutes to dissolve precipitated solids. DO NOT BOIL! Mix gently, but thoroughly to ensure the solution is homogeneous.

Load the Ladder onto gel and run.

Load volume: 

2-4ul of RNA Ladder per 5mm of mini gel lane width.

After the run is complete, stain the gel or perform Western transfer procedure as desired.

Note

When loading on gel use the same 2X Loading Dye Solution to both your RNA sample and RNA Ladder.

When visualizing a gel in UV light, additional dark zone of ethidium  bromide (in direction opposite to that of RNA) during electrophoresis. This, however, has no influence on the quality of RNA separation.

For optimal results, add ethidium bromide to both native agarose gel and electrophoresis buffer at the same final concentration of 0.5ug/ml. Run gels at 5V/cm. 

Caution

This product is extremely sensitive to degradation by ribonucleases. The use of diethyl pyrocarbonate-treated solutions and protective gloves is recommended.