CERTIFICATE OF ANALYSIS
RNA Ladder, High Range
(for 100 applications)
Lot: 9625 Quality guaranteed: 03.2010
Supplied with: 1ml 2X Loading Dye Solution
Store: at -70℃ or -20℃. (up to 6 months)
RNA Ladder, High range, is a set of seven RNA transcripts with lengths of 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200 bases. RNA transcripts are produced from specific templates, containing a fragment of the pTZ19R polylinker and λ phage sequences. The RNA Ladder Range is suitable for analysis of RNA in native or denaturing agarose gels and can be easily visualized by ethidium bromide staining.
20mM potassium acetate (pH 4.5)
2x Loading Dye Solution
95% formamide, 0.025% SDS,
0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, 0.5mM EDTA.
Quality Control Assay Data
Analysis of 1ug (2 ul)of RNA Ladder, High Range, on a native agarose gel with ethidium bromide staining generates a clear eight-band pattern.
Quality authorized by: Linda Sun
RECOMMENDATIONS FOR USE
Thaw the marker at room temperature or heat at 37-40℃ for a few minutes to dissolve precipitated solids. DO NOT BOIL! Mix gently, but thoroughly to ensure the solution is homogeneous.
Load the Ladder onto gel and run.
2-4ul of RNA Ladder per 5mm of mini gel lane width.
After the run is complete, stain the gel or perform Western transfer procedure as desired.
When loading on gel use the same 2X Loading Dye Solution to both your RNA sample and RNA Ladder.
When visualizing a gel in UV light, additional dark zone of ethidium bromide (in direction opposite to that of RNA) during electrophoresis. This, however, has no influence on the quality of RNA separation.
For optimal results, add ethidium bromide to both native agarose gel and electrophoresis buffer at the same final concentration of 0.5ug/ml. Run gels at 5V/cm.
This product is extremely sensitive to degradation by ribonucleases. The use of diethyl pyrocarbonate-treated solutions and protective gloves is recommended.
|Product Name||RNA Marker High Range|