Mix

CERTIFICATE OF ANALYSIS

MassRuler

DNA Ladder, Mix,

Ready-to-use

#65-0403

Lot:                   0600

Supplied with: 1ml 6x Mass Loading Dye Solution 

Store:   at room temperature or at +4℃ for periods up to 6 months

             For longer periods store at -20℃.

Description

MassRuler DNA Ladder, Mix, is designed for evaluation of an unknown DNA fragment's size and quantity in a single electrophoretic run.

The DNA Ladder contains the following 20 discrete fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80.

Three reference bands (10000, 1031 and 500bp) contain the largest amount of material (the highest intensity).

The DNA Ladder is dissolved in storage and loading buffer and can be directly applied onto an agarose gel.

Storage and Loading Buffer

10mM Tris-HCl (pH7.6), 10mM EDTA, 0.015% bromophenol blue and 10% glycerol.

6x Mass Loading Dye Solution

0.09% bromophenol blue, 60% glycerol and 60mM EDTA.

Quality Control Assay Data

Agrose gel electrophoretic analysis of homogeneity of restriction endonucleases fragmentation patterns. All bands are sharp and clearly distinguishable. 

Quantitative analysis of bands is performed by densitometric scan of the gel. The integrated intensities of the bands are calibrated against those of standard fragments run in parallel lanes and are accurate within 10%.

Quality authorized by: Jurgita Zilinskiene

RECOMMENDATIONS FOR USE

Vortex gently just prior to use

Do not heat before loading

For accurate quantification:

-load equal volumes of the DNA Ladder and an experimental sample.

-if  necessary, adjust the concentration of your sample such that the expected amount of material loaded is approximately equal to that of a DNA Ladder band of a nearest size.