CERTIFICATE OF ANALYSIS
DNA Ladder Mix
#65-0331 0.05mg (for 100 applications)
Concentration: 0.1mg DNA/ml
Supplied with: 1ml 6x Loading Dye Solution
Store: at room temperature at +4℃
For periods up to 6 months.
For longer periods store at -20℃.
GeneRuler 100bp DNA Ladder is supplied in gel loading buffer at total DNA concentration of 0.1ug/ul and can be directly applied on to agarose gel. This DNA Ladder is prepared from a specially designed plasmid pE-50 DNA, containing pUC, λ phage and yeast genome sequences.
The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1000, 900,800,700,600,500,400,300, 250,200,150,100,80.
Supplied in Gel Loading Buffer
10mM Tris-HCI (pH7.6), 10mM EDTA.
0.03%bromophenol blue, 0.03% xylene cyanol FF and 10% glycerol.
6x Loading Dye Solution
0.2% bromophenol blue, 0.2% xylene cyanol FF, 60% glycerol and 60mM EDTA.
Quality Control Assay Data
Agarose gel electrophoretic analysis of homogeneity of Eco 147l and Pvul fragmentation patterns.
RECOMMENDATIONS FOR USE
Vortex gently just prior to use.
Do not heat before loading
Apply 0.1ul (1ul) of DNA Ladder per 1mm of agarose gel lane width.
Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining.
GeneRuler 100bp DNA Ladder, ready-to-use, can be used for DNA quantification (see Table).
Table. The percent and ng quantities of DNA fragments in 1ug of GeneRuler 100bp DNA Ladder, ready-to-use.
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