• 1 kb Plus | Size Range: 100-15,000 bp | Qty: 600 µl
• High resolution, pre-mixed and ready-to-use, 6 μl per lane
• Nuclease-free and high purity to avoid contamination
• Clear, stable bands for easy visualization and size determination
CERTIFICATE OF ANALYSIS
GeneRuler
DNA Ladder Mix
Ready-to-use
#65-0331 0.05mg (for 100 applications)
Concentration: 0.1mg DNA/ml
Lot: 2000
Supplied with: 1ml 6x Loading Dye Solution
Store: at room temperature at +4℃
For periods up to 6 months.
For longer periods store at -20℃.
Description
GeneRuler 100bp DNA Ladder is supplied in gel loading buffer at total DNA concentration of 0.1ug/ul and can be directly applied on to agarose gel. This DNA Ladder is prepared from a specially designed plasmid pE-50 DNA, containing pUC, λ phage and yeast genome sequences.
The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1000, 900,800,700,600,500,400,300, 250,200,150,100,80.
Supplied in Gel Loading Buffer
10mM Tris-HCI (pH7.6), 10mM EDTA.
0.03%bromophenol blue, 0.03% xylene cyanol FF and 10% glycerol.
6x Loading Dye Solution
0.2% bromophenol blue, 0.2% xylene cyanol FF, 60% glycerol and 60mM EDTA.
Quality Control Assay Data
Agarose gel electrophoretic analysis of homogeneity of Eco 147l and Pvul fragmentation patterns.
RECOMMENDATIONS FOR USE:
• Vortex gently just prior to use
• Do not heat before loading
• Apply 0.1ul (1ul) of DNA Ladder per 1mm of agarose gel lane width
• Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining
• GeneRuler 100bp DNA Ladder, ready-to-use, can be used for DNA quantification
• The percent and ng quantities of DNA fragments in 1ug of GeneRuler 100bp DNA Ladder, ready-to-use.
Product Name | 1kb Plus DNA Ladder, 100-15,000 bp, 600 µl |
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SKU | 65-0331 |