1kb Plus DNA Ladder

CERTIFICATE OF ANALYSIS

GeneRuler

DNA Ladder Mix

Ready-to-use 

#65-0331 0.05mg (for 100 applications) 

Concentration: 0.1mg DNA/ml 

Lot:                  2000

Supplied with: 1ml 6x Loading Dye Solution 

Store:          at room temperature at +4℃ 

                     For periods up to 6 months. 

                     For longer periods store at -20℃. 

Description

GeneRuler 100bp DNA Ladder is supplied in gel loading buffer at total DNA concentration of 0.1ug/ul and can be directly applied on to agarose gel. This DNA Ladder is prepared from a specially designed plasmid pE-50 DNA, containing pUC, λ phage and yeast genome sequences. 

The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1000, 900,800,700,600,500,400,300, 250,200,150,100,80. 

Supplied in Gel Loading Buffer

10mM Tris-HCI (pH7.6), 10mM EDTA. 

0.03%bromophenol blue, 0.03% xylene cyanol FF and 10% glycerol. 

6x Loading Dye Solution

0.2% bromophenol blue, 0.2% xylene cyanol FF, 60% glycerol and 60mM EDTA. 

Quality Control Assay Data

Agarose gel electrophoretic analysis of homogeneity of Eco 147l and Pvul fragmentation patterns.  

RECOMMENDATIONS FOR USE:

• Vortex gently just prior to use
• Do not heat before loading 
• Apply 0.1ul (1ul) of DNA Ladder per 1mm of agarose gel lane width
• Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining
• GeneRuler 100bp DNA Ladder, ready-to-use, can be used for DNA quantification
• The percent and ng quantities of DNA fragments in 1ug of GeneRuler 100bp DNA Ladder, ready-to-use.