100bp

CERTIFICATE OF ANALYSIS

GeneRuler

100bp DNA Ladder  

#65-0241 0.05mg Ready-Load  

Lot: 3500   

Concentration: 0.05mg DNA/ml 

Supplied with:  1ml 6X Loading Dye Solution  

Store at -20 ℃  

Description

GeneRuler 100bp DNA Ladder is prepared from a specially designed plasmid pME-80J3 DNA, containing pUC, λ phage and yeast genome sequences. pME-80J3 DNA was completely digested with Eco1471 and Pvul. The DNA Ladder is dissolved in storage buffer. The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80.  

Storage Buffer

10mM Tris-HCI(pH7.6), 1mM EDTA.  

6X Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA.  

Quality Control Assay Data

Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1 ug (2ul)- on polyacrylamide gel by ethidium bromide staining generates 11 discrete bands pattern.  

Quality authorized by:        Jurgita Zilinskiene  

RECOMMENDATIONS FOR USE

Loading on agarose gel: 

-Prepare the DNA Ladder before loading: 

1ul (0.5ug) of the DNA Ladder, 

1ul of 6x Loading Dye Solution, 

4ul of deionized water; 

-vortex gently just prior to use; 

-do not heat before loading; 

-apply the prepared amount (6ul) of the DNA Ladder on a 5mm lane of agarose gel; 

-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.  

Note

• One vial 0.05mg) is sufficient for ~ 100 applications. 
• Use 0.1ug (0.2ul) of the DNA Ladder (before dilution) per 1mm of an agarose gel lane width.