100 bp DNA Ladder

CERTIFICATE OF ANALYSIS

GeneRuler

100bp DNA Ladder  

#65-0241 0.05mg Ready-Load  

Lot: 3500   

Concentration: 0.05mg DNA/ml 

Supplied with:  1ml 6X Loading Dye Solution  

Store at -20 ℃  

Description

GeneRuler 100bp DNA Ladder is prepared from a specially designed plasmid pME-80J3 DNA, containing pUC, λ phage and yeast genome sequences. pME-80J3 DNA was completely digested with Eco1471 and Pvul. The DNA Ladder is dissolved in storage buffer. The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80.  

Storage Buffer

10mM Tris-HCI(pH7.6), 1mM EDTA.  

6X Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA.  

Quality Control Assay Data

Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1 ug (2ul)- on polyacrylamide gel by ethidium bromide staining generates 11 discrete bands pattern.  

Quality authorized by:        Jurgita Zilinskiene  

RECOMMENDATIONS FOR USE:

• Loading on agarose gel:
     Prepare the DNA Ladder before loading: 
     1ul (0.5ug) of the DNA Ladder, 
     1ul of 6x Loading Dye Solution, 
     4ul of deionized water; 
• Vortex gently just prior to use
• Do not heat before loading 
• Apply the prepared amount (6ul) of the DNA Ladder on a 5mm lane of agarose gel
• Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining
• 1ug of the GeneRuler  1kb DNA Ladder, ready-to-use, can be contains ≡310ng (≡31%) of the 3000bp fragment. 

Note:

• One vial (0.05mg) is sufficient for ~ 100 applications
• Use 0.1ug (0.2ul) of the DNA Ladder (before dilution) per 1mm of an agarose gel lane width.