100 bp DNA Ladder, 80-1031 bp, 600 µl

SKU
65-0241
$99.00

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In stock

 

• 100 bp | Size Range: 80-1031 bp | Qty: 600 µl
• High resolution, pre-mixed and ready-to-use, 6 μl per lane
• Nuclease-free and high purity to avoid contamination
• Clear, stable bands for easy visualization and size determination

100 bp DNA Ladder

CERTIFICATE OF ANALYSIS

GeneRuler

100bp DNA Ladder  

#65-0241 0.05mg Ready-Load  

Lot: 3500   

Concentration: 0.05mg DNA/ml 

Supplied with:  1ml 6X Loading Dye Solution  

Store at -20 ℃  

Description

GeneRuler 100bp DNA Ladder is prepared from a specially designed plasmid pME-80J3 DNA, containing pUC, λ phage and yeast genome sequences. pME-80J3 DNA was completely digested with Eco1471 and Pvul. The DNA Ladder is dissolved in storage buffer. The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80.  

Storage Buffer

10mM Tris-HCI(pH7.6), 1mM EDTA.  

6X Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA.  

Quality Control Assay Data

Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1 ug (2ul)- on polyacrylamide gel by ethidium bromide staining generates 11 discrete bands pattern.  

Quality authorized by:        Jurgita Zilinskiene  

 

RECOMMENDATIONS FOR USE:

• Loading on agarose gel:
     Prepare the DNA Ladder before loading: 
     1ul (0.5ug) of the DNA Ladder, 
     1ul of 6x Loading Dye Solution, 
     4ul of deionized water; 
• Vortex gently just prior to use
• Do not heat before loading 
• Apply the prepared amount (6ul) of the DNA Ladder on a 5mm lane of agarose gel
• Following electrophoretic separation on agarose gel the DNA bands can be visualized by ethidium bromide staining
• 1ug of the GeneRuler  1kb DNA Ladder, ready-to-use, can be contains ≡310ng (≡31%) of the 3000bp fragment. 

Note:

• One vial (0.05mg) is sufficient for ~ 100 applications
• Use 0.1ug (0.2ul) of the DNA Ladder (before dilution) per 1mm of an agarose gel lane width. 

There is no safety sheet available for this product
More Information
Product Name 100 bp DNA Ladder, 80-1031 bp, 600 µl
SKU 65-0241
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